This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FGFR3_HUMAN_D0
catalog :
79731
citations: 2
Reference
Li A, Voleti R, Lee M, Gagoski D, Shah N. High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display. elife. 2023;12: pubmed publisher
Albanese S, Parton D, Işık M, Rodríguez Laureano L, Hanson S, Behr J, et al. An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression. Biochemistry. 2018;57:4675-4689 pubmed publisher
product information
Catalog Number :
79731
Product Name :
FGFR3_HUMAN_D0
article :
doi10.1021/acs.biochem.7b01081
id18654
pubmed_id30004690
bacterial resistance :
Ampicillin
cloning :
backbonepET His10 TEV LIC cloning vector (2B-T-10)
backbone_mutation
backbone_originScott Gradia (Addgene plasmid # 78173)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
growth strain :
This plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACH, CD333, CEK2, HSFGFR3EX, JTK4
geneFGFR3
id2261
genbank_ids
mutation
nameFGFR3
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis10-TEV
resistance markers :
2903
3190
3196
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA