This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DAPK3_HUMAN_D0
catalog :
79721
citations: 1
Reference
Albanese S, Parton D, Işık M, Rodríguez Laureano L, Hanson S, Behr J, et al. An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression. Biochemistry. 2018;57:4675-4689 pubmed publisher
product information
Catalog Number :
79721
Product Name :
DAPK3_HUMAN_D0
article :
doi10.1021/acs.biochem.7b01081
id18654
pubmed_id30004690
bacterial resistance :
Ampicillin
cloning :
backbonepET His10 TEV LIC cloning vector (2B-T-10)
backbone_mutation
backbone_originScott Gradia (Addgene plasmid # 78173)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
growth strain :
This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDLK, ZIP, ZIPK
geneDAPK3
id1613
genbank_ids
mutation
nameDAPK3
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis10-TEV
resistance markers :
2903
3190
3196
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA