MERTK_HUMAN_D0 | Addgene 79705 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MERTK_HUMAN_D0

catalog :

79705

citations: 3

Reference
Hayashi S, Craddock B, Miller W. Phosphorylation of Ack1 by the Receptor Tyrosine Kinase Mer. Kinases Phosphatases. 2023;1:167-180 pubmed publisher
Li A, Voleti R, Lee M, Gagoski D, Shah N. High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display. elife. 2023;12: pubmed publisher
Albanese S, Parton D, Işık M, Rodríguez Laureano L, Hanson S, Behr J, et al. An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression. Biochemistry. 2018;57:4675-4689 pubmed publisher

product information

Catalog Number :

79705

Product Name :

MERTK_HUMAN_D0

article :

doi	10.1021/acs.biochem.7b01081
id	18654
pubmed_id	30004690

bacterial resistance :

Ampicillin

cloning :

backbone

pET His10 TEV LIC cloning vector (2B-T-10)

backbone_mutation

backbone_origin

Scott Gradia (Addgene plasmid # 78173)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/

growth strain :

This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	T7
sequencing_primer_3	T7 reverse
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MER, RP38, Tyro12, c-Eyk, c-mer
gene	MERTK
id	10461

genbank_ids

mutation

name

MERTK

shRNA_sequence

size

species

9606

Homo sapiens