This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SRC_HUMAN_D0
catalog :
79700
citations: 2
Reference
Roy M, Surudoi M, Kropp A, Hou J, Dai W, Hardy J, et al. Structural mapping of PEAK pseudokinase interactions identifies 14-3-3 as a molecular switch for PEAK3 signaling. Nat Commun. 2023;14:3542 pubmed publisher
Albanese S, Parton D, Işık M, Rodríguez Laureano L, Hanson S, Behr J, et al. An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression. Biochemistry. 2018;57:4675-4689 pubmed publisher
product information
Catalog Number :
79700
Product Name :
SRC_HUMAN_D0
article :
doi10.1021/acs.biochem.7b01081
id18654
pubmed_id30004690
bacterial resistance :
Ampicillin
cloning :
backbonepET His10 TEV LIC cloning vector (2B-T-10)
backbone_mutation
backbone_originScott Gradia (Addgene plasmid # 78173)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
growth strain :
This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesASV, SRC1, THC6, c-SRC, p60-Src
geneSRC
id6714
genbank_ids
mutation
nameSRC
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis10-TEV
resistance markers :
2903
3190
3196
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA