MO70 - Orai1 EC-Flag | Addgene 79590 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

MO70 - Orai1 EC-Flag

catalog :

79590

citations: 1

Reference
Srikanth S, Jung H, Ribalet B, Gwack Y. The intracellular loop of Orai1 plays a central role in fast inactivation of Ca2+ release-activated Ca2+ channels. J Biol Chem. 2010;285:5066-75 pubmed publisher

product information

Catalog Number :

79590

Product Name :

MO70 - Orai1 EC-Flag

article :

doi	10.1074/jbc.M109.072736
id	18631
pubmed_id	20007711

bacterial resistance :

Ampicillin

cloning :

backbone

MO70

backbone_mutation

backbone_origin

backbone_size

7480

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

This plasmid contains a FLAG tag in its extracellular loop 2 between transmembranes 3 and 4. This plasmid can be used for surface staining to detect Orai1 expression on the plasma membrane. Please note that compared to GenBank reference sequence NP_116179.2, the Orai1 insert in this plasmid is missing two amino acids (P8 and P9). This difference does not affect the function, as the clone has been tested for functionality in Orai1 KO MEFs and can reconstitute Orai1 function in Orai1 KO cells identical to WT (untagged) Orai1. The functional reconstitution data are presented in the original JBC paper (PMID 20007711) describing this construct.

growth strain :

Transient expression and retroviral Expression

origin :

pi :

alt_names

TMEM142A

CRACM1

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	XhoI
promoter	CMV
sequencing_primer_3	pCDH-Rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CRACM1, IMD9, ORAT1, TAM2, TMEM142A
gene	ORAI1
id	84876

genbank_ids

mutation

name

Orai1

shRNA_sequence

size

901

species

9606

Homo sapiens