This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAc5.1B-EGFP-Dm4E-T
catalog :
79261
citations: 1
Reference
Igreja C, Peter D, Weiler C, Izaurralde E. 4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation. Nat Commun. 2014;5:4790 pubmed publisher
product information
Catalog Number :
79261
Product Name :
pAc5.1B-EGFP-Dm4E-T
article :
doi10.1038/ncomms5790
id18593
pubmed_id25179781
bacterial resistance :
Ampicillin
cloning :
backbonepAc5.1B-EGFP
backbone_mutation
backbone_originInvitrogen
backbone_size6071
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Schneider 2 cell Expression
growth strain :
Expresses EGFP-tagged Dm4E-T in S2 cells
origin :
37
pi :
alt_names
CG32016C
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoterAc5 promoter
sequencing_primer_3Bgh rev primer
sequencing_primer_5EGFP-forw
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG32016, CG11097, CG11232, CG32016, Dm4E-T, Dmel CG32016
gene4E-T
id43836
genbank_ids
mutation
nameDm4E-T
shRNA_sequence
size3033
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagEGFP
resistance markers :
223
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA