This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAc5.1B-EGFP-DmNot3
catalog :
79250
citations: 1
Reference
Haas G, Braun J, Igreja C, Tritschler F, Nishihara T, Izaurralde E. HPat provides a link between deadenylation and decapping in metazoa. J Cell Biol. 2010;189:289-302 pubmed publisher
product information
Catalog Number :
79250
Product Name :
pAc5.1B-EGFP-DmNot3
article :
doi10.1083/jcb.200910141
id3365
pubmed_id20404111
bacterial resistance :
Ampicillin
cloning :
backbonepAc5.1B-EGFP
backbone_mutationN-terminal EGFP-tag
backbone_originInvitrogen
backbone_size6065
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Schneider 2 cell Expression
growth strain :
Expresses EGFP-tagged DmNot3 in S2 cells
origin :
37
pi :
alt_names
CG8426
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5HindIII
promoterAc5 promoter
sequencing_primer_3Bgh rev primer
sequencing_primer_5EGFP-forw
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG8426, CG8426, CNOT3, Dmel CG8426, NOT3, NOT3.5, NOT3/5, l(2)NC136, not3, not3/5
geneNot3
id35501
genbank_ids
NM_136332
mutation
nameDmNot3
shRNA_sequence
size2535
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagEGFP
resistance markers :
223
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA