This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCOLA-T7-WspR:G249A
catalog :
79163
citations: 3
Reference
Manna S, Kellenberger C, Hallberg Z, Hammond M. Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors. Methods Mol Biol. 2021;2323:121-140 pubmed publisher
Yeo J, Wang X, Hammond M. Live Flow Cytometry Analysis of c-di-GMP Levels in Single Cell Populations. Methods Mol Biol. 2017;1657:111-130 pubmed publisher
Wang X, Wilson S, Hammond M. Next-generation RNA-based fluorescent biosensors enable anaerobic detection of cyclic di-GMP. Nucleic Acids Res. 2016;44:e139 pubmed publisher
product information
Catalog Number :
79163
Product Name :
pCOLA-T7-WspR:G249A
article :
doi10.1093/nar/gkw580
id22069
pubmed_id27382070
bacterial resistance :
Kanamycin
cloning :
backbonepCOLADuet
backbone_mutation
backbone_originNovagen
backbone_size3719
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses inactive WspR G249A under a T7 promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5NdeI
promoterT7
sequencing_primer_3gctagttattgctcagcgg
sequencing_primer_5ttgtacacggccgcataatc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPA3702
genewspR
id878337
genbank_ids
NC_002516.2
mutationG249A
nameWspR
shRNA_sequence
size1041
species
32644
Other
P. areuginosa
tags
resistance markers :
2906
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA