This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSicoR (EGFP) shPnky-2
catalog :
79142
citations: 1
Reference
Ramos A, Andersen R, Liu S, Nowakowski T, Hong S, Gertz C, et al. The long noncoding RNA Pnky regulates neuronal differentiation of embryonic and postnatal neural stem cells. Cell Stem Cell. 2015;16:439-447 pubmed publisher
product information
Catalog Number :
79142
Product Name :
pSicoR (EGFP) shPnky-2
article :
doi10.1016/j.stem.2015.02.007
id18571
pubmed_id25800779
bacterial resistance :
Ampicillin
cloning :
backbonepSicoR
backbone_mutation
backbone_origin
backbone_size7552
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
Cre/Lox
growth strain :
Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre
origin :
37
pi :
musculustags
lnc-pou3f2gened>Gm30731id1
05500046able>genbank_ids
mutation
nameshPnky-2
shRNA_sequenced>GATGACGTGGAGAGGATTTTTCAAGAGAAAATCCTCT
CCACGTCATCTTTTTTCsize
species
10090
Mus
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5HpaI
promoterU6 for the shRNA and CMV for EGFP
sequencing_primer_3
sequencing_primer_5tgcaggggaaagaatagtagac
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesPnky,
resistance markers :
2885
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA