This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSpyTag-GlpG
catalog :
79054
citations: 1
Reference
Min D, Arbing M, Jefferson R, Bowie J. A simple DNA handle attachment method for single molecule mechanical manipulation experiments. Protein Sci. 2016;25:1535-44 pubmed publisher
product information
Catalog Number :
79054
Product Name :
pSpyTag-GlpG
article :
doi10.1002/pro.2952
id18542
pubmed_id27222403
bacterial resistance :
Ampicillin
cloning :
backbonepTrcHisB
backbone_mutation
backbone_originInvitrogen
backbone_size4269
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Transform pSpyTag-GlpG plasmid into E. coli BL21-Gold (DE3) for expression.
growth strain :
Expresses SpyTagged GlpG protein
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5SacI
promoterTrc
sequencing_primer_3pBAD-R
sequencing_primer_5M13pUC-Rev
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb3424, ECK3410
geneglpG
id947936
genbank_ids
mutationGlpG protein employed here corresponds to residues 87-276 of E. coli GlpG.
nameSpyTag-GlpG
shRNA_sequence
size723
species
tags
locationN terminal on insert
tag6xHis
locationN terminal on insert
tagSpyTag
locationC terminal on insert
tagSpyTag
resistance markers :
655
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA