This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET30a-AlkB-D135S
catalog :
79051
citations: 10
Reference
Scheepbouwer C, Aparicio Puerta E, Gómez Martín C, Verschueren H, van Eijndhoven M, Wedekind L, et al. ALL-tRNAseq enables robust tRNA profiling in tissue samples. Genes Dev. 2023;37:243-257 pubmed publisher
Drino A, K xf6 nig L, Capitanchik C, Sanadgol N, Janisiw E, Rappol T, et al. Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs. Nucleic Acids Res. 2023;51:1326-1352 pubmed publisher
Chen Z, Zhu W, Zhu S, Sun K, Liao J, Liu H, et al. METTL1 promotes hepatocarcinogenesis via m7 G tRNA modification-dependent translation control. Clin Transl Med. 2021;11:e661 pubmed publisher
Tucker J, Schaller A, Willis I, Glaunsinger B. Alteration of the Premature tRNA Landscape by Gammaherpesvirus Infection. MBio. 2020;11: pubmed publisher
Cui J, Liu Q, Sendinc E, Shi Y, Gregory R. Nucleotide resolution profiling of m3C RNA modification by HAC-seq. Nucleic Acids Res. 2021;49:e27 pubmed publisher
Warren J, Salinas Giegé T, Hummel G, Coots N, Svendsen J, Brown K, et al. Combining tRNA sequencing methods to characterize plant tRNA expression and post-transcriptional modification. RNA Biol. 2021;18:64-78 pubmed publisher
Lin S, Liu Q, Jiang Y, Gregory R. Nucleotide resolution profiling of m7G tRNA modification by TRAC-Seq. Nat Protoc. 2019;14:3220-3242 pubmed publisher
Cosentino C, Toivonen S, Diaz Villamil E, Atta M, Ravanat J, Demine S, et al. Pancreatic β-cell tRNA hypomethylation and fragmentation link TRMT10A deficiency with diabetes. Nucleic Acids Res. 2018;46:10302-10318 pubmed publisher
Lin S, Liu Q, Lelyveld V, Choe J, Szostak J, Gregory R. Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Mol Cell. 2018;71:244-255.e5 pubmed publisher
Zheng G, Qin Y, Clark W, Dai Q, Yi C, He C, et al. Efficient and quantitative high-throughput tRNA sequencing. Nat Methods. 2015;12:835-837 pubmed publisher
product information
Catalog Number :
79051
Product Name :
pET30a-AlkB-D135S
article :
doi10.1038/nmeth.3478
id18538
pubmed_id26214130
bacterial resistance :
Kanamycin
cloning :
backbonepET30a
backbone_mutation
backbone_originNovagen
backbone_size5243
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Express mutant AlkB protein (D135S) in E. coli BL21(DE3)
growth temp :
BL21 (DE3) for expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesb2212, ECK2204, aidD
genealkB
id946708
genbank_ids
946708
mutationA truncated AlkB with deletion of the amino (N)-terminal 11 amino acids and D135S mutation
nameAlkB
shRNA_sequence
size615
species
32644
Other
E.coli
tags
locationN terminal on backbone
tagHis tag
resistance markers :
2929
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA