This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET30a-AlkB
catalog :
79050
citations: 12
Reference
Scheepbouwer C, Aparicio Puerta E, Gómez Martín C, Verschueren H, van Eijndhoven M, Wedekind L, et al. ALL-tRNAseq enables robust tRNA profiling in tissue samples. Genes Dev. 2023;37:243-257 pubmed publisher
Drino A, K xf6 nig L, Capitanchik C, Sanadgol N, Janisiw E, Rappol T, et al. Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs. Nucleic Acids Res. 2023;51:1326-1352 pubmed publisher
Bryan C, Wei X, Wang Z, Yang K. In vitro eradication of abasic site-mediated DNA-peptide/protein cross-links by Escherichia coli long-patch base excision repair. J Biol Chem. 2022;298:102055 pubmed publisher
Chen Z, Zhu W, Zhu S, Sun K, Liao J, Liu H, et al. METTL1 promotes hepatocarcinogenesis via m7 G tRNA modification-dependent translation control. Clin Transl Med. 2021;11:e661 pubmed publisher
Tucker J, Schaller A, Willis I, Glaunsinger B. Alteration of the Premature tRNA Landscape by Gammaherpesvirus Infection. MBio. 2020;11: pubmed publisher
Cui J, Liu Q, Sendinc E, Shi Y, Gregory R. Nucleotide resolution profiling of m3C RNA modification by HAC-seq. Nucleic Acids Res. 2021;49:e27 pubmed publisher
Lin S, Liu Q, Jiang Y, Gregory R. Nucleotide resolution profiling of m7G tRNA modification by TRAC-Seq. Nat Protoc. 2019;14:3220-3242 pubmed publisher
Schmidt L, Werner S, Kemmer T, Niebler S, Kristen M, Ayadi L, et al. Graphical Workflow System for Modification Calling by Machine Learning of Reverse Transcription Signatures. Front Genet. 2019;10:876 pubmed publisher
Zhou H, Rauch S, Dai Q, Cui X, Zhang Z, Nachtergaele S, et al. Evolution of a reverse transcriptase to map N1-methyladenosine in human messenger RNA. Nat Methods. 2019;16:1281-1288 pubmed publisher
Cosentino C, Toivonen S, Diaz Villamil E, Atta M, Ravanat J, Demine S, et al. Pancreatic β-cell tRNA hypomethylation and fragmentation link TRMT10A deficiency with diabetes. Nucleic Acids Res. 2018;46:10302-10318 pubmed publisher
Lin S, Liu Q, Lelyveld V, Choe J, Szostak J, Gregory R. Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Mol Cell. 2018;71:244-255.e5 pubmed publisher
Zheng G, Qin Y, Clark W, Dai Q, Yi C, He C, et al. Efficient and quantitative high-throughput tRNA sequencing. Nat Methods. 2015;12:835-837 pubmed publisher
product information
Catalog Number :
79050
Product Name :
pET30a-AlkB
article :
doi10.1038/nmeth.3478
id18538
pubmed_id26214130
bacterial resistance :
Kanamycin
cloning :
backbonepET30a
backbone_mutation
backbone_originNovagen
backbone_size5243
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Express wild type AlkB protein in E. coli BL21(DE3)
growth temp :
BL21 (DE3) for expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesb2212, ECK2204, aidD
genealkB
id946708
genbank_ids
946708
mutationA truncated AlkB with deletion of the amino (N)-terminal 11 amino acids
nameAlkB
shRNA_sequence
size615
species
32644
Other
E.coli
tags
locationN terminal on backbone
tagHis tag
resistance markers :
2929
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA