This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-TRSter
catalog :
78865
citations: 2
Reference
Zhang S, Zhou J, Fan Q, Lv X, Wang T, Wang F, et al. Discovery of hydroxytyrosol as thioredoxin reductase 1 inhibitor to induce apoptosis and G1/S cell cycle arrest in human colorectal cancer cells via ROS generation. Exp Ther Med. 2021;22:829 pubmed publisher
Arner E, Sarioglu H, Lottspeich F, Holmgren A, Bock A. High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS elements and co-expression with the selA, selB and selC genes. J Mol Biol. 1999;292:1003-16 pubmed
product information
Catalog Number :
78865
Product Name :
pET-TRSter
article :
doi10.1006/jmbi.1999.3085
id18543
pubmed_id10512699
bacterial resistance :
Kanamycin
cloning :
backbonepET-24(s)+
backbone_mutation
backbone_originNovagen Inc.
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Additional article references: PubMed PMID: 15345395 A > G (nucleotide) at bp 4 compared to NM_031614.2, which results in N2D compared to NP_113802.2.
growth strain :
Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
growth temp :
Expression host BL21(DE3)
origin :
37
pi :
alt_names
Txnrd1 (a.k.a. TR1 or TrxR1)
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NcoI
promoterT7lac
sequencing_primer_3T7 terminal
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesTr
geneTxnrd1
id58819
genbank_ids
NM_031614.2
mutation
nameThioredoxin Reductase
shRNA_sequence
size3450
species
10116
Rattus norvegicus
tags
location
tagMutated E. coli-type SECIS element (3 end of insert)
resistance markers :
2876
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA