This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HisMBP CFlm25
catalog :
78458
citations: 1
Reference
Coseno M, Martin G, Berger C, Gilmartin G, Keller W, DOUBLIE S. Crystal structure of the 25 kDa subunit of human cleavage factor Im. Nucleic Acids Res. 2008;36:3474-83 pubmed publisher
product information
Catalog Number :
78458
Product Name :
HisMBP CFlm25
article :
doi10.1093/nar/gkn079
id18372
pubmed_id18445629
bacterial resistance :
Ampicillin
cloning :
backbonepDEST-HisMBP fusion vector
backbone_mutation
backbone_originDavid Waugh, National Cancer Institute, NIH
backbone_size8282
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Has a TEV cleavage site.
growth strain :
Inducible expression in E. coli
origin :
37
pi :
alt_names
CPSF5
NUDT21
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promotertac
sequencing_primer_3M13-F20
sequencing_primer_5MBP-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFIM25, CPSF5
geneNUDT21
id11051
genbank_ids
11051
mutation
nameHuman Cleavage Factor lm 25 kDa subunit
shRNA_sequence
size681
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
locationN terminal on backbone
tagMBP
plasmid copy :
Original CFlm25 gene came from Greg Gilmartin (University of Vermont).
resistance markers :
2933
tags :
Low Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA