This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo
catalog :
78353
citations: 1
Reference
Bintu L, Yong J, Antebi Y, McCue K, Kazuki Y, Uno N, et al. Dynamics of epigenetic regulation at the single-cell level. Science. 2016;351:720-4 pubmed publisher
product information
Catalog Number :
78353
Product Name :
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo
article :
doi10.1126/science.aab2956
id18274
pubmed_id26912859
bacterial resistance :
Ampicillin
cloning :
backbonepExchange1
backbone_mutationoriginal CMV was replaced with pEF cut with BamHI and KpnI before Gibson inserted Zeo in the loxP site using Cre
backbone_originAgilent Technologies
backbone_size6615
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth strain :
Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
origin :
30
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterpEF alpha
sequencing_primer_3T7 primer: TAATACGACTCACTATAGGG
sequencing_primer_5F1-factor: CTAAAGTGCGAAAGCGGCGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID
geneHDAC4
id9759
genbank_ids
https://www.ncbi.nlm.nih.gov/gene/9759
mutation
nameH2B-mCherry-T2A-rTetR-HDAC4
shRNA_sequence
size5040
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagrTetR
plasmid copy :
HDAC4 Flag was purchased from Addgene (https://www.addgene.org/13821/); rTetR was PCR-amplified from the rtTA3 system, Clontech
resistance markers :
861
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA