pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4 | Addgene 78349 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

catalog :

78349

citations: 2

Reference
Noviello G, Gjaltema R, Schulz E. CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression. Nat Commun. 2023;14:3225 pubmed publisher
Bintu L, Yong J, Antebi Y, McCue K, Kazuki Y, Uno N, et al. Dynamics of epigenetic regulation at the single-cell level. Science. 2016;351:720-4 pubmed publisher

product information

Catalog Number :

78349

Product Name :

pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

article :

doi	10.1126/science.aab2956
id	18274
pubmed_id	26912859

bacterial resistance :

Ampicillin

cloning :

backbone

pExchange1

backbone_mutation

original CMV was replaced with pEF cut with BamHI and KpnI before Gibson

backbone_origin

Agilent Technologies

backbone_size

4116

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Synthetic Biology

growth strain :

Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	pEF alpha
sequencing_primer_3	T7 primer: TAATACGACTCACTATAGGG
sequencing_primer_5	F1-factor: CTAAAGTGCGAAAGCGGCGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID
gene	HDAC4
id	9759

genbank_ids

https://www.ncbi.nlm.nih.gov/gene/9759

mutation

name

H2B-mCherry-T2A-rTetR-HDAC4

shRNA_sequence

size

5040

species

9606

Homo sapiens

100

Synthetic