pTpal-fdc | Addgene 78288 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTpal-fdc

catalog :

78288

citations: 1

Reference
McKenna R, Pugh S, Thompson B, Nielsen D. Microbial production of the aromatic building-blocks (S)-styrene oxide and (R)-1,2-phenylethanediol from renewable resources. Biotechnol J. 2013;8:1465-75 pubmed publisher

product information

Catalog Number :

78288

Product Name :

pTpal-fdc

article :

doi	10.1002/biot.201300035
id	18362
pubmed_id	23801570

bacterial resistance :

Ampicillin

cloning :

backbone

pTrc99A

backbone_mutation

backbone_origin

backbone_size

4176

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

*Note: Addgene's quality control sequencing finds an E50A amino acid residue substitution in the A. thaliana PAL2 gene insert. The depositing laboratory does not believe this residue change will affect protein function. This plasmid accurately reflects the construct as it was used in the associated publication.

growth strain :

Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	NcoI
promoter	trc
sequencing_primer_3	TCTAGATTAGCAAATCGGAA
sequencing_primer_5	acacaggaaacagaccatgg
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT3G53260, ATPAL2, phenylalanine ammonia-lyase 2
gene	PAL2
id	824493

genbank_ids

mutation

*see below

name

PAL2

shRNA_sequence

size

2154

species

3702

Arabidopsis thaliana