pSpal2At | Addgene 78286 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSpal2At

catalog :

78286

citations: 3

Reference
Grubbe W, Rasor B, Kr xfc ger A, Jewett M, Karim A. Cell-free styrene biosynthesis at high titers. Metab Eng. 2020;61:89-95 pubmed publisher
Crook N, Ferreiro A, Gasparrini A, Pesesky M, Gibson M, Wang B, et al. Adaptive Strategies of the Candidate Probiotic E. coli Nissle in the Mammalian Gut. Cell Host Microbe. 2019;25:499-512.e8 pubmed publisher
McKenna R, Nielsen D. Styrene biosynthesis from glucose by engineered E. coli. Metab Eng. 2011;13:544-54 pubmed publisher

product information

Catalog Number :

78286

Product Name :

pSpal2At

article :

doi	10.1016/j.ymben.2011.06.005
id	18361
pubmed_id	21722749

bacterial resistance :

Chloramphenicol

cloning :

backbone

pSTV28

backbone_mutation

backbone_origin

backbone_size

2999

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

*Note: Addgene's quality control sequencing finds an E50A amino acid residue substitution in the A. thaliana PAL2 gene insert. The depositing laboratory does not believe this residue change will affect protein function. This plasmid accurately reflects the construct as it was used in the associated publication.

growth strain :

Expresses A. thaliana PAL2 in E. coli

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SphI
cloning_site_5	EcoRI
promoter	lac
sequencing_primer_3	CCAGTGCCAAGCTTGCATGC
sequencing_primer_5	ctatgaccatgattacgaattc
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT3G53260, ATPAL2, phenylalanine ammonia-lyase 2
gene	PAL2
id	824493

genbank_ids

mutation

*see below

name

PAL2

shRNA_sequence

size

2154

species

3702

Arabidopsis thaliana