This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-dCas9-D3A
catalog :
78256
citations: 2
Reference
Bar Sadeh B, Pnueli L, Keestra S, Bentley G, Melamed P. Srd5a1 is Differentially Regulated and Methylated During Prepubertal Development in the Ovary and Hypothalamus. J Endocr Soc. 2023;7:bvad108 pubmed publisher
McDonald J, Celik H, Rois L, Fishberger G, Fowler T, Rees R, et al. Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation. Biol Open. 2016;5:866-74 pubmed publisher
product information
Catalog Number :
78256
Product Name :
pCMV-dCas9-D3A
article :
doi10.1242/bio.019067
id18482
pubmed_id27170255
bacterial resistance :
Ampicillin
cloning :
backbonepCMV
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5NheI
promoterpCMV
sequencing_primer_3ATGCTCGTCAAGAAGACAGGGCCAGGTTTC
sequencing_primer_5ctgcagccttcaagtacttcgacacc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
AF480163
mutation
nameDNMT3A CD aa 598-912
shRNA_sequence
size948
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag epitope tag
resistance markers :
2842
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA