This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TetO-dCas9-mD3A
catalog :
78255
citations: 1
Reference
McDonald J, Celik H, Rois L, Fishberger G, Fowler T, Rees R, et al. Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation. Biol Open. 2016;5:866-74 pubmed publisher
product information
Catalog Number :
78255
Product Name :
TetO-dCas9-mD3A
article :
doi10.1242/bio.019067
id18482
pubmed_id27170255
bacterial resistance :
Ampicillin
cloning :
backboneAddgene # 20321
backbone_mutationWe took out OSKM and put in new MCS site: EcoRI-XbaI-NheI-AgeI-PspXI-AscI-EcoRI
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5NheI
promoterCMV
sequencing_primer_3ATGCTCGTCAAGAAGACAGGGCCAGGTTTC
sequencing_primer_5ctgcagccttcaagtacttcgacacc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
AF480163
mutationE756A
nameDNMT3A CD aa 598-912
shRNA_sequence
size948
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag epitope tag
resistance markers :
2842
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA