pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

catalog :

78251

citations: 1

Reference
Don E, Formella I, Badrock A, Hall T, Morsch M, Hortle E, et al. A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Zebrafish. 2017;14:69-72 pubmed publisher

product information

Catalog Number :

78251

Product Name :

pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

article :

doi	10.1089/zeb.2016.1321
id	22483
pubmed_id	27631880

bacterial resistance :

Ampicillin

cloning :

backbone

pTol2pA2

backbone_mutation

backbone_origin

Chien Lab

backbone_size

4097

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Zebrafish motor neuron inducible expression

growth notes :

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

growth strain :

Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

origin :

pi :

alt_names

mnx1, Hb9

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	-3mnx1
sequencing_primer_3	pBluescript-SK [5'-tctagaactagtggatc-3']
sequencing_primer_5	Tol2-F [5'-CAATCCTGCAGTGCTGAAAA-3']
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	hlxb, hlxb9, zgc:112174
gene	mnx1
id	405399

genbank_ids

mutation

name

-3mnx1

shRNA_sequence

size

4809

species

7955

Danio rerio