This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTol2p2A--3mnx1:mTagBFP-NS-MCS
catalog :
78250
citations: 1
Reference
Don E, Formella I, Badrock A, Hall T, Morsch M, Hortle E, et al. A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Zebrafish. 2017;14:69-72 pubmed publisher
product information
Catalog Number :
78250
Product Name :
pTol2p2A--3mnx1:mTagBFP-NS-MCS
article :
doi10.1089/zeb.2016.1321
id22483
pubmed_id27631880
bacterial resistance :
Ampicillin
cloning :
backbonepTol2pA2
backbone_mutation
backbone_originChien Lab
backbone_size4143
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish motor neuron expression
growth notes :
Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
growth strain :
Can be used to subclone in gene of interest into blue motor neuron expression vector.
origin :
37
pi :
alt_names
mnx1, Hb9
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter-3mnx1
sequencing_primer_3EBV-rev [5'-GTGGTTTGTCCAAACTCATC-3']
sequencing_primer_5Tol2-F [5'-CAATCCTGCAGTGCTGAAAA-3']
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseshlxb, hlxb9, zgc:112174
genemnx1
id405399
genbank_ids
mutation
name-3mnx1
shRNA_sequence
size3742
species
7955
Danio rerio
tags
alt_names
cloning
entrez_gene
genbank_ids
mutation
name-mTagBFP-NS-MCS
shRNA_sequence
size
species
32644
Other
tags
resistance markers :
2850
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA