This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEx1-pEF-H2B-mCherry-T2A-rTetR-EED
catalog :
78101
citations: 1
Reference
Bintu L, Yong J, Antebi Y, McCue K, Kazuki Y, Uno N, et al. Dynamics of epigenetic regulation at the single-cell level. Science. 2016;351:720-4 pubmed publisher
product information
Catalog Number :
78101
Product Name :
pEx1-pEF-H2B-mCherry-T2A-rTetR-EED
article :
doi10.1126/science.aab2956
id18274
pubmed_id26912859
bacterial resistance :
Ampicillin
cloning :
backbonepExchange1
backbone_mutationoriginal CMV was replaced with pEF cut with BamHI and KpnI before Gibson
backbone_originAgilent Technologies
backbone_size4113
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth strain :
Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterpEF alpha
sequencing_primer_3T7 primer: TAATACGACTCACTATAGGG
sequencing_primer_5F1-factor: CTAAAGTGCGAAAGCGGCGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCOGIS, HEED, WAIT1
geneEED
id8726
genbank_ids
https://www.ncbi.nlm.nih.gov/gene/8726
mutation
nameH2B-mCherry-T2A-rTetR-EED
shRNA_sequence
size3114
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagrTetR
plasmid copy :
pCMV-HA-EED was purchased from Addgene (https://www.addgene.org/24231/); rTetR was PCR-amplified from the rtTA3 system, Clontech
resistance markers :
861
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA