This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins
catalog :
78099
citations: 5
Reference
Lensch S, Herschl M, Ludwig C, Sinha J, Hinks M, Mukund A, et al. Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells. elife. 2022;11: pubmed publisher
Muldoon J, Kandula V, Hong M, Donahue P, Boucher J, Bagheri N, et al. Model-guided design of mammalian genetic programs. Sci Adv. 2021;7: pubmed publisher
Van M, Fujimori T, Bintu L. Nanobody-mediated control of gene expression and epigenetic memory. Nat Commun. 2021;12:537 pubmed publisher
Donahue P, Draut J, Muldoon J, Edelstein H, Bagheri N, Leonard J. The COMET toolkit for composing customizable genetic programs in mammalian cells. Nat Commun. 2020;11:779 pubmed publisher
Bintu L, Yong J, Antebi Y, McCue K, Kazuki Y, Uno N, et al. Dynamics of epigenetic regulation at the single-cell level. Science. 2016;351:720-4 pubmed publisher
product information
Catalog Number :
78099
Product Name :
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins
article :
doi10.1126/science.aab2956
id18274
pubmed_id26912859
bacterial resistance :
Ampicillin
cloning :
backbonepSLR-test
backbone_mutationreplaced the SLR gene with Neo (blunt-end ligation after digesting pSLR-test with EcoRV/SmaI, see PLoS One 6.2 (2011): e17267) introduced MCS with 2 cHS4 insulators on each side
backbone_originToyobo, Osaka, Japan
backbone_size9432
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth notes :
Can be used for constitutive expression of H2B-citrine. Can be silenced by TetR-KRAB or rTetR-KRAB (using doxycycline). It can be site-specifically integrated in a PhiC31 attP site (the plasmid contains a PhiC31 attB site). The reporter gene is flanked by two full length (1.2kb each) chicken HS4 insulators on each side.
growth strain :
Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
origin :
37
pi :
alt_names
histone cluster 2, H2be
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter5xTetO pEF alpha
sequencing_primer_3TCIR_5 GGAGCTCCACCCTAGAACTA
sequencing_primer_5TCIF_3 GAAGACGTCGATATACGCGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE
geneH2BC21
id8349
genbank_ids
https://www.ncbi.nlm.nih.gov/gene/8349
mutation
nameHistone H2B - Citrine
shRNA_sequence
size3080
species
9606
Homo sapiens
100
Synthetic
tags
plasmid copy :
PhiC31-Neo-ins-MCS-ins was a gift from Mitsuo Oshimura. pCS-H2B-citrine was a gift from Sean Megasson.
resistance markers :
861
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA