This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ZAP70 gRNA (BRDN0001148252)
catalog :
77955
citations: 1
Reference
Doench J, Fusi N, Sullender M, Hegde M, Vaimberg E, Donovan K, et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016;34:184-191 pubmed publisher
product information
Catalog Number :
77955
Product Name :
ZAP70 gRNA (BRDN0001148252)
article :
doi10.1038/nbt.3437
id16517
pubmed_id26780180
bacterial resistance :
Ampicillin
cloning :
backbonelentiGuide-Puro
backbone_mutation
backbone_originZhang Lab (Addgene plasmid # 52963)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
The gRNA was designed to target the following Genbank reference sequence(s): NM_001079.3. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
growth strain :
3rd generation lentiviral gRNA plasmid targeting human ZAP70
origin :
37
pi :
alt_names
NM_001079.3
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterhU6
sequencing_primer_3pS2-R
sequencing_primer_5U6-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70
geneZAP70
id7535
genbank_ids
mutation
nameZAP70
shRNA_sequenceCGAGCGCAAACTTTACTCTG
size
species
tags
resistance markers :
2061
423
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA