CSF1R gRNA (BRDN0001146959) | Addgene 77036 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CSF1R gRNA (BRDN0001146959)

catalog :

77036

citations: 1

Reference
Doench J, Fusi N, Sullender M, Hegde M, Vaimberg E, Donovan K, et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016;34:184-191 pubmed publisher

product information

Catalog Number :

77036

Product Name :

CSF1R gRNA (BRDN0001146959)

article :

doi	10.1038/nbt.3437
id	16517
pubmed_id	26780180

bacterial resistance :

Ampicillin

cloning :

backbone

lentiGuide-Puro

backbone_mutation

backbone_origin

Zhang Lab (Addgene plasmid # 52963)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

CRISPR

growth notes :

The gRNA was designed to target the following Genbank reference sequence(s): NM_001288705.1,NM_005211.3. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.

growth strain :

3rd generation lentiviral gRNA plasmid targeting human CSF1R

origin :

pi :

alt_names

NM_001288705.1

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	hU6
sequencing_primer_3	pS2-R
sequencing_primer_5	U6-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BANDDOS, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, GPSC, HDLS, HDLS1, M-CSF-R
gene	CSF1R
id	1436

genbank_ids

mutation

name

CSF1R

shRNA_sequence

ACGCTACCTTCCAAAACACG

size

species

9606

Homo sapiens