EIF2AK2 gRNA (BRDN0001148577) | Addgene 75638 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

EIF2AK2 gRNA (BRDN0001148577)

catalog :

75638

citations: 1

Reference
Doench J, Fusi N, Sullender M, Hegde M, Vaimberg E, Donovan K, et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016;34:184-191 pubmed publisher

product information

Catalog Number :

75638

Product Name :

EIF2AK2 gRNA (BRDN0001148577)

article :

doi	10.1038/nbt.3437
id	16517
pubmed_id	26780180

bacterial resistance :

Ampicillin

cloning :

backbone

lentiGuide-Puro

backbone_mutation

backbone_origin

Zhang Lab (Addgene plasmid # 52963)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

CRISPR

growth notes :

The gRNA was designed to target the following Genbank reference sequence(s): NM_001135651.2,NM_001135652.2,NM_002759.3. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.

growth strain :

3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

origin :

pi :

alt_names

NM_001135651.2

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	hU6
sequencing_primer_3	pS2-R
sequencing_primer_5	U6-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DYT33, EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR
gene	EIF2AK2
id	5610

genbank_ids

mutation

name

EIF2AK2

shRNA_sequence

GCAACCTACCTCCTATCATG

size

species

9606

Homo sapiens