This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pYFP-RPB1aAmr
catalog :
75284
citations: 1
Reference
Darzacq X, Shav Tal Y, de Turris V, Brody Y, Shenoy S, Phair R, et al. In vivo dynamics of RNA polymerase II transcription. Nat Struct Mol Biol. 2007;14:796-806 pubmed
product information
Catalog Number :
75284
Product Name :
pYFP-RPB1aAmr
article :
doi10.1038/nsmb1280
id18220
pubmed_id17676063
bacterial resistance :
Kanamycin
cloning :
backbonepEYFP-C1
backbone_mutation
backbone_originBD Biosciences Clontech
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells
origin :
37
pi :
alt_names
RPB1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3pX-C1 REVseq TATGTTTCAGGTTCAGGGGG
sequencing_primer_5CMV Forward CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
genePOLR2A
id5430
genbank_ids
NM_000937.4
mutationN792D alpha-amanitin resistant
namePOLR2A
shRNA_sequence
size5911
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEYFP
resistance markers :
1896
880
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA