pMSCV40-EF1a-hBCL10(1-185)-SO | Addgene 75258 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMSCV40-EF1a-hBCL10(1-185)-SO

catalog :

75258

citations: 1

Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed

product information

Catalog Number :

75258

Product Name :

pMSCV40-EF1a-hBCL10(1-185)-SO

article :

doi	10.1074/jbc.M116.739326
id	22309
pubmed_id	27637333

bacterial resistance :

Ampicillin

cloning :

backbone

pMSCV-IRES-GFP (pMIG)

backbone_mutation

Added EF1a promoter prior to insert, added SV40-ori

backbone_origin

backbone_size

8400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

EF1a promoter ensures enhanced transgene transcription in some cells, but significantly reduces virus titer. This may result in less transduced cells, which produce high amounts of transgene, though, and can be sorted via GFP.

growth strain :

retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)

origin :

pi :

alt_names

BCL-10, CIPER

B-cell CLL/lymphoma 10

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	swaI
promoter	pMSCV-LTRs, EF1a
sequencing_primer_3	AGCTTGATATCGAATTCCG
sequencing_primer_5	TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CARMEN, CIPER, CLAP, IMD37, c-E10, mE10
gene	BCL10
id	8915

genbank_ids

8915 (gene-id)

NM_003921.4

mutation

AS 1-185 (w/o C-terminus)

name

Bcl10

shRNA_sequence

size

650

species

9606

Homo sapiens