This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pQCXIX_sh_hMIB2_#9
catalog :
75255
citations: 1
Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed
product information
Catalog Number :
75255
Product Name :
pQCXIX_sh_hMIB2_#9
article :
doi10.1074/jbc.M116.739326
id22309
pubmed_id27637333
bacterial resistance :
Ampicillin
cloning :
backbonepQCXIX (modified)
backbone_mutationincludes EF1a-EGFP expression cassette, mU6-shRNA expression cassette
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
Produced >90 % KD (Western Blot) in Jurkat cells after RV-transduction and sort for GFPpositive cells.
growth strain :
retroviral shRNA vector against human MIB2, expresses GFP for transduction control
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5HpaI
promotermU6
sequencing_primer_3
sequencing_primer_5CCC TAA CTG TAA AGT AAT TG
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesZZANK1, ZZZ5
geneMIB2
id142678
genbank_ids
142678 (Gene-ID)
mutation
nameshRNA against MIB2 (human)
shRNA_sequenceGGAAGGTGGTGAAAGTGTT
size
species
9606
Homo sapiens
tags
resistance markers :
2819
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA