pQCXIX_sh_hBcl10_#1 | Addgene 75248 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pQCXIX_sh_hBcl10_#1

catalog :

75248

citations: 1

Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed

product information

Catalog Number :

75248

Product Name :

pQCXIX_sh_hBcl10_#1

article :

doi	10.1074/jbc.M116.739326
id	22309
pubmed_id	27637333

bacterial resistance :

Ampicillin

cloning :

backbone

pQCXIX (modified)

backbone_mutation

includes EF1a-EGFP expression cassette, mU6-shRNA expression cassette

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

RNAi

growth notes :

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. Produced 70-80 % KD (Western Blot) in Jurkat cells after RV-transduction and sort for GFPpositive cells.

growth strain :

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

origin :

pi :

alt_names

BCL-10, CIPER

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	HpaI
promoter	mU6
sequencing_primer_3
sequencing_primer_5	CCC TAA CTG TAA AGT AAT TG
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	CARMEN, CIPER, CLAP, IMD37, c-E10, mE10
gene	BCL10
id	8915

genbank_ids

8915 (gene-id)

mutation

name

shRNA against hBcl10

shRNA_sequence

GAACAACCTCTCCAGATCA

size

species

9606

Homo sapiens