Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | Addgene 75240 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

catalog :

75240

citations: 1

Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed

product information

Catalog Number :

75240

Product Name :

Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

article :

doi	10.1074/jbc.M116.739326
id	22309
pubmed_id	27637333

bacterial resistance :

Ampicillin

cloning :

backbone

PX458-pSpCas9(BB)2A-GFP

backbone_mutation

CBH promoter exchanged for hEF1a promoter

backbone_origin

Feng Zhang (Plasmid #48138)

backbone_size

9703

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

CRISPR

growth notes :

gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhance specificity (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237 43, 1 p following 243 (2015).) targets all isoforms.

growth strain :

CRISPR/Cas9 plasmid against human Ikaros

origin :

pi :

alt_names

IKZF1

cloning

entrez_gene

aliases	CVID13, Hs.54452, IK1, IKAROS, LYF1, LyF-1, PPP1R92, PRO0758, ZNFN1A1
gene	IKZF1
id	10320

genbank_ids

10320 (Gene-ID)

mutation

name

sgRNA against human Ikaros

shRNA_sequence

(gg)TCTGGAGTATCGCTTACAGG

size

species

9606

Homo sapiens