NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

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product summary

company name :

Addgene

product type :

cDNA

product name :

NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

catalog :

75234

citations: 1

Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed

product information

Catalog Number :

75234

Product Name :

NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

article :

doi	10.1074/jbc.M116.739326
id	22309
pubmed_id	27637333

bacterial resistance :

Ampicillin

cloning :

backbone

PX461-pSpCas9n(BB)2A-GFP

backbone_mutation

CBH promoter exchanged for hEF1a promoter

backbone_origin

Feng Zhang (Plasmid #48140)

backbone_size

9703

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

CRISPR

growth notes :

gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237 43, 1 p following 243 (2015).) targets all isoforms. MUST BE COTRANSFECTED WITH PLASMID #75235

growth strain :

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

origin :

pi :

alt_names

NFAT1

cloning

entrez_gene

aliases	JCOSL, NFAT1, NFATP
gene	NFATC2
id	4773

genbank_ids

4773 (GENE-ID)

mutation

name

sgRNA against NFATc2

shRNA_sequence

(gg)GCCGCAGAAGTTTCTGAGCG

size

species

9606

Homo sapiens