This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AcpP pet29a C-terminal His Tag
catalog :
75016
citations: 1
Reference
Worthington A, Rivera H, Torpey J, Alexander M, Burkart M. Mechanism-based protein cross-linking probes to investigate carrier protein-mediated biosynthesis. ACS Chem Biol. 2006;1:687-91 pubmed
product information
Catalog Number :
75016
Product Name :
AcpP pet29a C-terminal His Tag
article :
doi10.1021/cb6003965
id18172
pubmed_id17184132
bacterial resistance :
Kanamycin
cloning :
backbonepet29a
backbone_mutation
backbone_origin
backbone_size5371
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase
growth temp :
For protein expression, grow in BL21s at 37 degrees until an OD of 0.8 is reached (approximately 3-4 hours), induce with 0.5 mM IPTG and grow at 16 degrees for 16 hours.
origin :
37
pi :
alt_names
Acyl carrier protein from E. coli fatty acid synthase
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3GCTAGTTATTGCTCAGCGG (T7 terminator, reverse primer)
sequencing_primer_5TAATACGACTCACTATAGGG (T7 promoter, forward primer)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb1094, ECK1080
geneacpP
id944805
genbank_ids
944805
NC_000913.3
mutation
nameAcpP
shRNA_sequence
size238
species
32644
Other
E. coli
tags
locationC terminal on backbone
tagHis Tag
resistance markers :
2803
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA