This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCIG1_UL136_23kDa_Myc
catalog :
74949
citations: 1
Reference
Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F. Complex expression of the UL136 gene of human cytomegalovirus results in multiple protein isoforms with unique roles in replication. J Virol. 2014;88:14412-25 pubmed publisher
product information
Catalog Number :
74949
Product Name :
pCIG1_UL136_23kDa_Myc
article :
doi10.1128/JVI.02711-14
id18158
pubmed_id25297993
bacterial resistance :
Ampicillin
cloning :
backbonepCIG1
backbone_mutation
backbone_origin
backbone_size7535
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Cloned by Dr. Katie Caviness. Expresses ONLY the 23kda isoform, expression of all other isoforms has been suppressed by mutating initiating methionine residues to alanine. Amino acids 1-99 are not present in this clone.
growth strain :
Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.
origin :
37
pi :
alt_names
HCMV UL136 protein, subcloned from TB40E
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV, Lentiviral LTR
sequencing_primer_3pCDH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHHV5wtgp125
geneUL136
id3077568
genbank_ids
EF999921.1
ABV71655.1
mutationdelta 1-99 M128A
nameUL136
shRNA_sequence
size453
species
32644
Other
Human Cytomegalovirus strain TB40/E
tags
locationC terminal on insert
tagHA Tag (YPYDVPDYA)
resistance markers :
2808
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA