This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCX-TRI-2A
catalog :
74674
citations: 1
Reference
Cinti A, De Giorgi M, Chisci E, Arena C, Galimberti G, Farina L, et al. Simultaneous Overexpression of Functional Human HO-1, E5NT and ENTPD1 Protects Murine Fibroblasts against TNF-α-Induced Injury In Vitro. PLoS ONE. 2015;10:e0141933 pubmed publisher
product information
Catalog Number :
74674
Product Name :
pCX-TRI-2A
article :
doiPONE-D-15-33437 [pii]
id18100
pubmed_id26513260
bacterial resistance :
Kanamycin
cloning :
backbonepCX-2A-2A
backbone_mutation
backbone_originGiovannoni lab, Addgene plasmid 74670
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Contains a D473N mutation in CD73, but it does not impair either the plasmid function or the hCD73 protein activity.
growth strain :
mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5EcoRI
promoterpCAGGS
sequencing_primer_3
sequencing_primer_5pCAX-F CAGCTCCTGGGCAACGTGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHMOX1D, HO-1, HSP32, bK286B10
geneHMOX1
id3162
genbank_ids
mutation
nameHO1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagF2A
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5AflII
promoter
sequencing_primer_3
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT
geneNT5E
id4907
genbank_ids
mutation
nameCD73
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagF2A
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI
promoter
sequencing_primer_3M13R
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATPDase, CD39, NTPDase-1, SPG64
geneENTPD1
id953
genbank_ids
mutation
nameCD39
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
HMOX1 (hHO-1) sequence was kindly provided by Prof. Fritz Bach's laboratory at Harvard Medical School, Boston. NT5E (hCD73) sequence was kindly provided by Dr. Jozef Spychala at University of Michigan, Ann Arbor. ENTPD1 (hCD39) sequence was kindly provided by Dr. Simon Robson at Harvard Medical School, Boston.
resistance markers :
2784
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA