This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcFLAG-CSK-R107E
catalog :
74503
citations: 1
Reference
Kazi J, Vaapil M, Agarwal S, Bracco E, PÄhlman S, Ronnstrand L. The tyrosine kinase CSK associates with FLT3 and c-Kit receptors and regulates downstream signaling. Cell Signal. 2013;25:1852-60 pubmed publisher
product information
Catalog Number :
74503
Product Name :
pcFLAG-CSK-R107E
article :
doi10.1016/j.cellsig.2013.05.016
id18061
pubmed_id23707526
bacterial resistance :
Ampicillin
cloning :
backbonepcFLAG
backbone_mutation
backbone_originthis lab, modified from pcDNA3
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The depositor notes that a BamHI restriction site was inserted following the start codon of CSK. This site does not affect plasmid function.
growth strain :
Overexpression of CSK R107E mutant in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterCMV
sequencing_primer_3BGHrev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCSK
id1445
genbank_ids
mutationR107E, K222R
nameCSK
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagFLAG
resistance markers :
2779
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA