This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pX462-hPRKAA2-gRNA_A
catalog :
74376
citations: 2
Reference
Chu X, Xiao X, Wang G, Uosef A, Lou X, Arnold P, et al. Gasdermin D-mediated pyroptosis is regulated by AMPK-mediated phosphorylation in tumor cells. Cell Death Dis. 2023;14:469 pubmed publisher
Toyama E, Herzig S, Courchet J, Lewis T, Losón O, Hellberg K, et al. Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress. Science. 2016;351:275-281 pubmed publisher
product information
Catalog Number :
74376
Product Name :
pX462-hPRKAA2-gRNA_A
article :
doi10.1126/science.aab4138
id18008
pubmed_id26816379
bacterial resistance :
Ampicillin
cloning :
backbonepX462
backbone_mutation
backbone_origin Addgene plasmid 48141
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth strain :
gRNA_A to knockout human AMPK alpha 2 using Cas9n.
origin :
37
pi :
alt_names
PRKAA2
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterU6
sequencing_primer_3
sequencing_primer_5GAGGGCCTATTTCCCATGATTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesAMPK, AMPK2, AMPKa2, PRKAA
genePRKAA2
id5563
genbank_ids
mutation
namehuman AMPK alpha 2 exon1 gRNA
shRNA_sequenceGTCAGCCATCTTCGGCGCGCG
size
species
9606
Homo sapiens
tags
resistance markers :
463
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA