14UAS PSD95:GFP 5UAS DSRedExpress | Addgene 74315 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

14UAS PSD95:GFP 5UAS DSRedExpress

catalog :

74315

citations: 2

Reference
DeMarco E, Stoner G, Robles E. A genetic labeling system to study dendritic spine development in zebrafish models of neurodevelopmental disorders. Dis Model Mech. 2022;15: pubmed publisher
Niell C, Meyer M, Smith S. In vivo imaging of synapse formation on a growing dendritic arbor. Nat Neurosci. 2004;7:254-60 pubmed

product information

Catalog Number :

74315

Product Name :

14UAS PSD95:GFP 5UAS DSRedExpress

article :

doi	10.1038/nn1191
id	17995
pubmed_id	14758365

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-N2

backbone_mutation

CMV replaced by 14 UAS repeats

backbone_origin

clontech/invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

growth notes :

The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.

growth strain :

Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

origin :

pi :

alt_names

cloning

entrez_gene

genbank_ids

mutation

Y599H and I793S (please see depositor comment below)

name

zebrafish PSD95

shRNA_sequence

size

2406

species

7955

Danio rerio