This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
NRCoC
catalog :
74300
citations: 2
Reference
Velichko A, Ovsyannikova N, Petrova N, Luzhin A, Vorobjeva M, Gavrikov A, et al. Treacle and TOPBP1 control replication stress response in the nucleolus. J Cell Biol. 2021;220: pubmed publisher
Winokur S, Shiang R. The Treacher Collins syndrome (TCOF1) gene product, treacle, is targeted to the nucleolus by signals in its C-terminus. Hum Mol Genet. 1998;7:1947-52 pubmed
product information
Catalog Number :
74300
Product Name :
NRCoC
article :
doiddb229 [pii]
id17989
pubmed_id9811939
bacterial resistance :
Kanamycin
cloning :
backbonepS65T-C1
backbone_mutation
backbone_originClontech
backbone_size4728
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Addgene sequencing found extra LVPNSCSPGDPLVLERPAVPSTVPRARDPPDLDN on the C-terminus of the Tcof1 translation.
growth strain :
Mammalian expression of full-length treacle fused to GFP
origin :
37
pi :
alt_names
Tcof1
cloning
clone_methodRestriction Enzyme
cloning_site_3PstI
cloning_site_5unknown
promoterCMV
sequencing_primer_3pEGFP-R
sequencing_primer_5GFP-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasestreacle
geneTcof1
id21453
genbank_ids
mutation
nametreacle ribosome biogenesis factor 1
shRNA_sequence
size1302
species
10090
Mus musculus
tags
locationN terminal on backbone
tagGFP
resistance markers :
2894
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA