This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNCS-mClover3
catalog :
74236
citations: 4
Reference
Ogino Y, Ansai S, Watanabe E, Yasugi M, Katayama Y, Sakamoto H, et al. Evolutionary differentiation of androgen receptor is responsible for sexual characteristic development in a teleost fish. Nat Commun. 2023;14:1428 pubmed publisher
Aregger M, Lawson K, Billmann M, Costanzo M, Tong A, Chan K, et al. Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism. Nat Metab. 2020;2:499-513 pubmed publisher
Schlechter R, Jun H, Bernach M, Oso S, Boyd E, Muñoz Lintz D, et al. Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria. Front Microbiol. 2018;9:3052 pubmed publisher
Bajar B, Wang E, Lam A, Kim B, Jacobs C, Howe E, et al. Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Sci Rep. 2016;6:20889 pubmed publisher
product information
Catalog Number :
74236
Product Name :
pNCS-mClover3
article :
doi10.1038/srep20889
id17967
pubmed_id26879144
bacterial resistance :
Ampicillin
cloning :
backbonepNCS
backbone_mutation
backbone_origin
backbone_size2916
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The specific substitutions used in the development of mClover3 relative to its parent Clover are N149Y, G160C, and A206K.
growth strain :
Constitutive expression of mClover3 in bacteria
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7 Xpress Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namemClover3
shRNA_sequence
size717
species
tags
locationN terminal on backbone
tag6xHis
resistance markers :
1358
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA