This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMXspuro-GFP-NBR1-dUBA
catalog :
74205
citations: 1
Reference
Kenific C, Stehbens S, Goldsmith J, Leidal A, Faure N, Ye J, et al. NBR1 enables autophagy-dependent focal adhesion turnover. J Cell Biol. 2016;212:577-90 pubmed publisher
product information
Catalog Number :
74205
Product Name :
pMXspuro-GFP-NBR1-dUBA
article :
doi10.1083/jcb.201503075
id17950
pubmed_id26903539
bacterial resistance :
Ampicillin
cloning :
backbonepMXs-puro
backbone_mutationInsert was digested with BglII/XhoI to ligate into the vector which was digested with BamHI/XhoI.
backbone_origin
backbone_size6900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Retroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codon
origin :
37
pi :
alt_names
NBR1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5GACGGCATCGCAGCTTGGATACAC
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases1A1-3B, IAI3B, M17S2, MIG19
geneNBR1
id4077
genbank_ids
mutationChanged aa 877 to encode for premature stop codon
nameneighbor of BRCA1 gene 1
shRNA_sequence
size2631
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
plasmid copy :
GFP-NBR1 was cloned from pMXs-IP-GFP-NBR1, a gift from Noboru Mizushima (Addgene plasmid # 38283)
resistance markers :
636
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA