This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLCKO_Luciferase_sgRNA
catalog :
74190
citations: 2
Reference
Mair B, Aregger M, Tong A, Chan K, Moffat J. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9. Methods Mol Biol. 2022;2377:1-27 pubmed publisher
Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown K, Macleod G, et al. High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015;163:1515-26 pubmed publisher
product information
Catalog Number :
74190
Product Name :
pLCKO_Luciferase_sgRNA
article :
doi10.1016/j.cell.2015.11.015
id16484
pubmed_id26627737
bacterial resistance :
Ampicillin
cloning :
backbonepLCKO
backbone_mutation
backbone_originMoffat Lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth strain :
lentiviral vector expressing sgRNA targeting Luciferase
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BfuAI
cloning_site_5BfuAI
promoterU6 Promoter
sequencing_primer_3
sequencing_primer_5U6 fwd
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
mutation
nameLuciferase sgRNA
shRNA_sequenceACAACTTTACCGACCGCGCC
size
species
32644
Other
tags
resistance markers :
721
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA