This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28a-PHGDH
catalog :
74113
citations: 1
Reference
Mullarky E, Lucki N, Beheshti Zavareh R, Anglin J, Gomes A, Nicolay B, et al. Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers. Proc Natl Acad Sci U S A. 2016;113:1778-83 pubmed publisher
product information
Catalog Number :
74113
Product Name :
pET28a-PHGDH
article :
doi10.1073/pnas.1521548113
id17913
pubmed_id26831078
bacterial resistance :
Kanamycin
cloning :
backbonepET-28a
backbone_mutation
backbone_origin
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expression of N-terminal His-tagged
origin :
37
pi :
alt_names
pET28A-PHGDH
phosphoglycerate dehydrogenase
cloning
clone_methodRestriction Enzyme
cloning_site_3Ndel
cloning_site_5Ndel
promoterT7 promoter
sequencing_primer_3T7 Terminator
sequencing_primer_5T7 Promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliases3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA
genePHGDH
id26227
genbank_ids
26227
mutation
namePHGDH
shRNA_sequence
size1033
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis-tagged
plasmid copy :
Vector backbone was a gift from M. G. Vander Heiden Lab
resistance markers :
64
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA