This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIG-AVITEV- hNFATc1/aA
catalog :
74057
citations: 1
Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed
product information
Catalog Number :
74057
Product Name :
pMIG-AVITEV- hNFATc1/aA
article :
doi10.1074/jbc.M116.739326
id22309
pubmed_id27637333
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV-IRES-GFP (pMIG)
backbone_mutationadded SV40-ORI
backbone_origin
backbone_size7200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The Avi-Tev-Tag is biotinylated by BirA E.coli biotin ligase when coexpressed, and can be cleaved by TEV protease.
growth strain :
retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site
origin :
37
pi :
alt_names
NFAT2
cloning
clone_methodRestriction Enzyme
cloning_site_3Sgf1
cloning_site_5SwaI, SnaB1
promoterpMSCV-LTRs
sequencing_primer_3AGCTTGATATCGAATTCCG
sequencing_primer_5CGT TCG ACC CCG CCT CGA TCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNF-ATC, NF-ATc1.2, NFAT2, NFATc
geneNFATC1
id4772
genbank_ids
NM_172390.2
mutationmutation G1157A [R235Q] and the silent mutation C1980T, as already described in the original publication Northrop, J. P. et al. Nature 369, 497 502 (1994).
namehuman NFATc1, isoform alpha-A
shRNA_sequence
size2250
species
9606
Homo sapiens
tags
locationN terminal on insert
tagAVI-TEV
plasmid copy :
Human NFATC1/ A sequence was sub-cloned from pREP-NFAT2, which was a gift from Anjana Rao (Addgene Plasmid #11788)
resistance markers :
2819
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA