pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV | Addgene 74055 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

catalog :

74055

citations: 1

Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed

product information

Catalog Number :

74055

Product Name :

pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

article :

doi	10.1074/jbc.M116.739326
id	22309
pubmed_id	27637333

bacterial resistance :

Ampicillin

cloning :

backbone

pMSCV-IRES-GFP (pMIG)

backbone_mutation

added SV40 ori

backbone_origin

backbone_size

7200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

The Avi-Tev-Tag is biotinylated by BirA E.coli biotin ligase when coexpressed, and can be cleaved by TEV protease. RIT mutation as described for mouse NFAT in Macian et al, EMBO J. (2000), 19, issue 17, p4783ff.

growth strain :

retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site

origin :

pi :

alt_names

NFAT1

nuclear factor of activated T-cells 2

cloning

clone_method	Restriction Enzyme
cloning_site_3	SnaB1, Sgf1
cloning_site_5	SacII
promoter
sequencing_primer_3	AGCTTGATATCGAATTCCG
sequencing_primer_5	CGTTCGACCCCGCCTCGATCC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	JCOSL, NFAT1, NFATP
gene	NFATC2
id	4773

genbank_ids

NM_173091.3

Gene-ID: 4773

mutation

silent mutation A1723C in the sequence of NM_173091.3 (A1503C position in ORF of the vector); RIT mutations (R466A-I467A-T533A) abbrogating AP-1 interaction, AS1-686 (deleted C-terminus)

name

human NFATc2, isoform C

shRNA_sequence

size

2150

species

9606

Homo sapiens

32644

Other

AS 1-686 (N terminus deleted)