This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIG-hNFATc2/C(1-686)AVITEV
catalog :
74054
citations: 1
Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed
product information
Catalog Number :
74054
Product Name :
pMIG-hNFATc2/C(1-686)AVITEV
article :
doi10.1074/jbc.M116.739326
id22309
pubmed_id27637333
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV-IRES-GFP (pMIG)
backbone_mutationadded SV40 ori
backbone_origin
backbone_size7200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The Avi-Tev-Tag is biotinylated by BirA E.coli biotin ligase when coexpressed, and can be cleaved by TEV protease.
growth strain :
retroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site
origin :
37
pi :
alt_names
NFAT1
nuclear factor of activated T-cells 2
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaB1, Sgf1
cloning_site_5SacII
promoterpMSCV-LTRs
sequencing_primer_3AGCTTGATATCGAATTCCG
sequencing_primer_5CGTTCGACCCCGCCTCGATCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesJCOSL, NFAT1, NFATP
geneNFATC2
id4773
genbank_ids
NM_173091.3
Gene-ID: 4773
mutationsilent mutation A1723C in the sequence of NM_173091.3 (A1503C position in ORF of the vector)
namehuman NFATc2, isoform C
shRNA_sequence
size2150
species
9606
Homo sapiens
32644
Other
AS 1-686 (N terminus deleted)
tags
locationC terminal on insert
tagAVI-TEV
resistance markers :
2819
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA