This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIG-hH4-AVITEV
catalog :
74051
citations: 1
Reference
Gabriel C, Gross F, Karl M, Stephanowitz H, Hennig A, Weber M, et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem. 2016;291:24172-24187 pubmed
product information
Catalog Number :
74051
Product Name :
pMIG-hH4-AVITEV
article :
doi10.1074/jbc.M116.739326
id22309
pubmed_id27637333
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV-IRES-GFP (pMIG)
backbone_mutationSV40 ori added
backbone_origin
backbone_size7200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Please note- there are several nucleotide mismatches when compared to NM_003540.3. This construct was codon-optomized to allow for high expression rates. The Avi-Tev-Tag is biotinylated by BirA E.coli biotin ligase when coexpressed, and can be cleaved by TEV protease.
growth strain :
retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaB1, Sgf1
cloning_site_5SacII
promoterpMSCV-LTRs
sequencing_primer_3AGCTTGATATCGAATTCCG
sequencing_primer_5CGTTCGACCCCGCCTCGATCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH4-16, H4/p, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, HIST4H4
geneH4C16
id121504
genbank_ids
NM_003540.3
mutation
namehuman Histone-H4
shRNA_sequence
size300
species
9606
Homo sapiens
tags
locationC terminal on insert
tagAVI-TEV
plasmid copy :
synthesis by company
resistance markers :
2819
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA