This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FH95pUp95bGW (B5)
catalog :
74014
citations: 1
| Reference |
|---|
Schlüter O, Xu W, Malenka R. Alternative N-terminal domains of PSD-95 and SAP97 govern activity-dependent regulation of synaptic AMPA receptor function. Neuron. 2006;51:99-111 pubmed
|
product information
Catalog Number :
74014
Product Name :
FH95pUp95bGW (B5)
article :
| doi | 10.1016/j.neuron.2006.05.016 |
| id | 8102 |
| pubmed_id | 16815335 |
bacterial resistance :
Ampicillin
cloning :
| backbone | FUGW | |||
| backbone_mutation | ||||
| backbone_origin | David Baltimore (Addgene plasmid # 14883) | |||
| backbone_size | 9941 | |||
| promoter | ||||
| sequencing_primer_3 | ||||
| sequencing_primer_5 | ||||
| vector_types |
|
growth notes :
Map: FHUG+W-HinDIII-SalI-XbaI-(p95b)-KpnI-SacII-PspOMI-SmaI-BamHI-(GFP)-BglII-BsrBI-EcoRV-HinDIII-ClaI Also associated with Liu, et al. J Neurophysiol. 2014 Feb;111(3):648-58. doi: 10.1152/jn.00262.2013 PMID 24225540. Psd95 shRNA expressed under control of H1 promoter. Psd95 coding sequence modified to GGATATTCAAGCGCATAAG make it resistant to the shRNA. Psd95-EGFP fusion protein expressed under control of UbC promoter. The publication by Schluter et al linked above describes the creation of the beta isoform of Psd95 in detail
growth strain :
lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion and Psd95 shRNA
origin :
37
pi :
|
resistance markers :
| 1006 |
| 3385 |
| 2752 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments