This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLEX-MCS-ASC-GFP
catalog :
73957
citations: 5
Reference
de Dios C, Abadin X, Roca Agujetas V, Jimenez Martinez M, Morales A, Trullas R, et al. Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis. Transl Neurodegener. 2023;12:10 pubmed publisher
Hsu C, Chávez C, Zhang C, Sowden M, Yan C, Berk B. The lipid peroxidation product 4-hydroxynonenal inhibits NLRP3 inflammasome activation and macrophage pyroptosis. Cell Death Differ. 2022;: pubmed publisher
Ernst O, Sun J, Lin B, Banoth B, Dorrington M, Liang J, et al. A genome-wide screen uncovers multiple roles for mitochondrial nucleoside diphosphate kinase D in inflammasome activation. Sci Signal. 2021;14: pubmed publisher
Ming S, Zeng L, Guo Y, Zhang S, Li G, Ma Y, et al. The Human-Specific STING Agonist G10 Activates Type I Interferon and the NLRP3 Inflammasome in Porcine Cells. Front Immunol. 2020;11:575818 pubmed publisher
de Almeida L, Khare S, Misharin A, Patel R, Ratsimandresy R, Wallin M, et al. The PYRIN Domain-only Protein POP1 Inhibits Inflammasome Assembly and Ameliorates Inflammatory Disease. Immunity. 2015;43:264-76 pubmed publisher
product information
Catalog Number :
73957
Product Name :
pLEX-MCS-ASC-GFP
article :
doi10.1016/j.immuni.2015.07.018
id17874
pubmed_id26275995
bacterial resistance :
Ampicillin
cloning :
backbonepLEX-Myc
backbone_mutationA MCS was inserted into the BamHI and XhoI sites (the BamH1 site was destroyed) as indicated in the attached map
backbone_origin
backbone_size11088
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Express human ASC with a C-terminal EGFP tag in mammalian cells
origin :
37
pi :
alt_names
ASC
TMS-1
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5Spe1
promoterCMV IE
sequencing_primer_3
sequencing_primer_5CMV iE FwD (CGCAAATGGGCGGTAGGCGTG)
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesASC, CARD5, TMS, TMS-1, TMS1
genePYCARD
id29108
genbank_ids
NM_013258.4
mutation
namePYD and CARD domain containing, PYCARD
shRNA_sequence
size2000
species
9606
Homo sapiens
tags
locationC terminal on insert
tagEGFP
resistance markers :
2763
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA